Human CRTAM ELISA Kit (ab267645)
Key features and details
- Sensitivity: 0.6 ng/ml
- Range: 0.614 ng/ml - 150 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CRTAM ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.6 ng/ml -
Range
0.614 ng/ml - 150 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 93.76 84% - 101% Plasma 114.6 93% - 126% Cell culture media 123.1 110% - 132% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CRTAM ELISA Kit is designed for the quantitative determination of CRTAM in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human CRTAM coated on a 96-well plate. Standards and samples are pipetted into the wells and CRTAM present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CRTAM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CRTAM bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE-2, Albumin, AMICA, ANG-4, BAFF, CA19-9, CD163 , Clusterin, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, aFGF, FOLR1, Furin, GASP-1, GASP-2, GCSF R, HAI-2, IL-17B R, IL-27, LAG-3 , LDL R , Pepsinogen I, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, TSP-1, TRAIL R2 , TRANCE, Troponin I, uPA, VE-Cadherin, WISP-1.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Anti-Human CRTAM coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human CRTAM Detection Antibody 2 vials Human CRTAM Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. -
Tissue specificity
In the immune system, expression is restricted to activated class-I MHC-restricted cells, including NKT and CD8 cells. Strongly expressed in spleen, thymus, small intestine, peripheral blood leukocyte, and in Purkinje neurons in cerebellum. Expressed at much lower levels in testis, ovary, colon, lung and lymphoid tissues. -
Sequence similarities
Belongs to the nectin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD355
- Class I MHC restricted T cell associated molecule
- Class-I MHC-restricted T-cell-associated molecule
see all -
Database links
- Entrez Gene: 56253 Human
- GenBank: BC070266.1 Human
- Omim: 612597 Human
- SwissProt: O95727 Human
- Unigene: 159523 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.