Anti-RBP1 antibody (ab31106)
Key features and details
- Goat polyclonal to RBP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBP1 antibody
See all RBP1 primary antibodies -
Description
Goat polyclonal to RBP1 -
Host species
Goat -
Specificity
Ab31106 recognises RBP1. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow
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Immunogen
Synthetic peptide:
VEGVVCKQVFKKVQ
, corresponding to C terminal amino acids 121-134 of Human PRBPI -
Positive control
- NIH 3T3 lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Ab31106 underwent ammonium sulphate precipitation prior to antigen affinity chromatography purification. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RBP1 antibody (ab31106)Lane 1 : Anti-RBP1 antibody (ab31106) at 2 µg/ml
Lane 2 : Anti-RBP1 antibody (ab31106) at 0.5 µg/ml
Lane 1 : NIH3T3 cell lysate
Lane 2 : U251 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-RBP1 antibody (ab31106)Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr with ab31106 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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Western blot - Anti-RBP1 antibody (ab31106)Anti-RBP1 antibody (ab31106) at 0.1 µg/ml + NIH 3T3 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
