Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14256(B)] to CRABP2
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CRABP2 antibody [EPR14256(B)]
See all CRABP2 primary antibodies
Description

Rabbit monoclonal [EPR14256(B)] to CRABP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- MCF7 cell lysate; Human skin lysate; Human liver and kidney lysates; MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14256(B)
Isotype

IgG
Research areas

Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 16 kDa
Observed band size: 16 kDa
Western blot - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Developed using the ECL technique.

Predicted band size: 16 kDa
Observed band size: 16 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
Flow Cytometry - Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Flow cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.
Anti-CRABP2 antibody [EPR14256(B)] (ab181255)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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