Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14256(B)] to CRABP2 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free
    See all CRABP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR14256(B)] to CRABP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250468 is the carrier-free version of ab181255.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/50000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    This data was developed using ab181255, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Western blot - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Anti-CRABP2 antibody [EPR14256(B)] (ab181255) at 1/10000 dilution + Human skin tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Developed using the ECL technique.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    This data was developed using ab181255, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human kidney tissue labeling CRABP2 with ab181255 at a 1/100 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Immunocytochemistry - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    This data was developed using ab181255, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 with ab181255 at a 1/250 dilution. As secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) (green) was used at a 1/200 dilution. DAPI staining in blue.
  • Flow Cytometry - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Flow Cytometry - Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    This data was developed using ab181255, the same antibody clone in a different buffer formulation.Flow cytometry analysis of, paraformaldehyde-fixed, MCF7 cells labeling CRABP2 (red) with ab181255 at a 1/80 dilution, or negative control rabbit monoclonal IgG (green). As secondary antibody goat anti rabbit IgG (FITC) was used at a 1/150 dilution.
  • Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)
    Anti-CRABP2 antibody [EPR14256(B)] - BSA and Azide free (ab250468)

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