Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)
![Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.com/ps/products/109/ab109523/Images/ab109523-122449-anti-noxa2-p67phox-antibody-epr5065-western-blot.jpg "Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5065] to NOXA2/p67phox
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NOXA2/p67phox antibody [EPR5065]
See all NOXA2/p67phox primary antibodies -
Description
Rabbit monoclonal [EPR5065] to NOXA2/p67phox -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- THP-1 or HL-60 treated with Dexomethasone and TPA cell lysate Human colon tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5065 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)All lanes : Anti-NOXA2/p67phox antibody [EPR5065] (ab109523) at 1/10000 dilution
Lane 1 : THP-1 cell lysate
Lane 2 : HL-60 treated with Dexomethasone and TPA cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 60 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.com/ps/products/109/ab109523/Images/ab109523-122448-anti-noxa2-p67phox-antibody-epr5065-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)ab109523, at a 1/500 dilution, staining Human NOXA2/p67phox in colon, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.com/ps/products/109/ab109523/Images/ab109523-122447-anti-noxa2-p67phox-antibody-epr5065-flow-cytometry.jpg)
Flow Cytometry - Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)Flow cytometry analysis of permeabilized THP-1 cells using ab109523 (red) and a rabbit IgG (negative contol) (green). The dilution is 1:150. The secondary antibody is a goat anti-rabbit IgG FITC. -
![Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)](https://www.abcam.com/ps/products/109/ab109523/Images/ab109523-4-benefits-of-recombinant-antibodies.png)
Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)
