Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CNTF ELISA Kit (ab264608)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 6.42 pg/ml
  • Range: 46.88 pg/ml - 3000 pg/ml
  • Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CNTF ELISA Kit
    See all CNTF kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 3.6%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 2.8%

  • Sample type

    Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    6.42 pg/ml

  • Range

    46.88 pg/ml - 3000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 85% - 94%
    Cell culture media 99 99% - 105%
    Hep Plasma 93 89% - 97%
    EDTA Plasma 93 87% - 105%
    Cit plasma 91 90% - 92%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human CNTF ELISA Kit (ab264608) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CNTF protein in cell culture media, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CNTF with 6.42 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Human Ciliary neurotrophic factor (CNTF) is a single chain polypeptide of 22.7 kDa. CNTF has been found to be a member of a limited family of neuropoietic cytokines that also includes Leukemia Inhibitory Factor and Oncostatin M. This family of cytokines are characterized by four distinctive α-helical folds commonly associated with growth hormone. CNTF has been hypothesized to be a target-derived differentiation factor for developing neurons and glial cells. CNTF that is retained intracellularly has been suggested to be a lesion-associated molecule, providing damaged neurons with trophic or survival support following injury. Human CNTF shows 76.6% and 77.4% sequence homology with mouse and rat, respectively.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of human CNTF standard curve in 1X Sample Diluent PTR.
    Example of human CNTF standard curve in 1X Sample Diluent PTR.

    The CNTF standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of spiked CNTF in human serum, plasma and cell culture supernatant samples.
    Interpolated concentrations of spiked CNTF in human serum, plasma and cell culture supernatant samples.

    The concentrations of CNTF were measured in duplicates, interpolated from the CNTF standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 12.5%, plasma (EDTA) 12.5%, plasma (heparin) 12.5% and cell culture media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Sandwich ELISA - Human CNTF ELISA Kit (ab264608)
    Sandwich ELISA - Human CNTF ELISA Kit (ab264608)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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