Human CLEC4C ELISA Kit (ab277413)
Key features and details
- Sensitivity: 0.082 ng/ml
- Range: 0.082 ng/ml - 20 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CLEC4C ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.082 ng/ml -
Range
0.082 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87.73 72% - 97% Plasma 82.11 75% - 87% Cell culture media 105.7 71% - 135% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CLEC4C ELISA Kit (ab277413) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CLEC4C in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human CLEC4C coated on a 96-well plate. Standards and samples are pipetted into the wells and Human CLEC4C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human CLEC4C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human CLEC4C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human CLEC2C Antibody 2 vials Anti-Human CLEC4C coated Microplate 1 unit Human CLEC4C standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Involved in antigen-capturing. Target ligand into antigen processing and peptide-loading compartments for presentation to T-cells. May mediate potent inhibition of induction of IFN-alpha/beta expression in plasmacytoid dendritic cells. May act as a signaling receptor that activates protein-tyrosine kinases and mobilizes intracellular calcium. Does not seem to bind mannose. -
Tissue specificity
Expressed in plasmacytoid dendritic cells (PDCs). Constitutively expressed in immature monocyte-derived dendritic cells (iMDDC) and is significantly down-regulated upon maturation with LPS but not with TNF-alpha. -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- BDCA-2
- BDCA2
- Blood dendritic cell antigen 2
see all -
Database links
- Entrez Gene: 170482 Human
- Omim: 606677 Human
- SwissProt: Q8WTT0 Human
- Unigene: 351812 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.