Human CKM ELISA Kit (ab185988)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 14 U/ml
- Range: 30 U/ml - 2000 U/ml
- Sample type: Cell culture extracts, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CKM ELISA Kit
See all Creatine Kinase MM kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Human serum 4 3% Inter-assay Sample n Mean SD CV% Human serum 3 9% -
Sample type
Urine, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
14 U/ml -
Range
30 U/ml - 2000 U/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 98 87% - 114% Serum 89 82% - 99% Plasma 115 93% - 140% Extraction Buffer 91 72% - 101% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CKM ELISA kit has been re-developed. We have identified new recombinant monoclonal antibodies to provide improved performance and consistency. This version will be discontinued when inventory is depleted. The new version is available as ab264617.
Human CKM ELISA Kit (ab185988) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CKM protein in edta plasma, hep plasma, serum, urine, cell culture extracts, tissue extracts, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CKM with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Creatine kinase (CK), also known as creatine phosphokinase (CPK) or phospho-creatine kinase, is an enzyme that catalyzes the reversible transfer of a high-energy phosphate group between phosphocreatine and ATP. There are five known CK isozymes, three located in the cytosol (CKMM, CKMB, CKBB) and two in the mitochondria (MiSCK and MiuCK). Structurally, cytosolic CK is a 86kDa dimeric enzyme of two identical or non-identical chains, CKM and/or CKB whereas mitochondrial CK is an octameric enzyme associated with mitochondrial membranes. The compartmentation of CK isoenzymes allows for direct association with ATP-providing or consuming processes both linked via metabolite channeling through the creatine phosphate shuttle. Thus CK isoenzymes allow for the generation of a large pool of phosphocreatine, considered to be a temporal and spatial ATP buffering system in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X CKM Capture Antibody 1 x 600µl 10X CKM Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human CKM Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Cellular localization
Cytoplasmic -
Alternative names
- CKM
- CKMM
- creatine kinase M chain
see all -
Database links
- Entrez Gene: 1158 Human
- Omim: 123310 Human
- SwissProt: P06732 Human
- Unigene: 334347 Human
Images
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Other - Human CKM ELISA Kit (ab185988)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of CKM standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of CKM standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Ten individual healthy donors were evaluated for the presence of CKM in serum using this assay.Results were interpolated from the standard curve in Sample Diluent NS and corrected by sample dilution (50 fold). The mean level of CKM is 115.3 U/L with a range of 54.3 – 268.9 U/L and a standard deviation of 77.8U/L.
