Human CEBPB ELISA Kit (ab233631)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CEBPB ELISA Kit
See all CEBP Beta kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rec protein 8 2.6% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
26 pg/ml -
Range
31.25 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 101.8 100.3% - 103.7% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CEBPB ELISA Kit (ab233631) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CEBPB protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CEBPB with 26 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CEBPB Capture Antibody 1 x 600µl 10X Human CEBPB Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human CEBPB Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Important transcriptional activator in the regulation of genes involved in immune and inflammatory responses. Specifically binds to an IL-1 response element in the IL-6 gene. NF-IL6 also binds to regulatory regions of several acute-phase and cytokines genes. It probably plays a role in the regulation of acute-phase reaction, inflammation and hemopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Functions in brown adipose tissue (BAT) differentiation. -
Tissue specificity
Expressed at low levels in the lung, kidney and spleen. -
Sequence similarities
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsSumoylated by polymeric chains of SUMO2 or SUMO3. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- AGP/EBP
- C EBP beta
- C/EBP beta
see all -
Database links
- Entrez Gene: 1051 Human
- Omim: 189965 Human
- SwissProt: P17676 Human
- Unigene: 517106 Human
- Unigene: 719041 Human
- Unigene: 720603 Human
Images
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Other - Human CEBPB ELISA Kit (ab233631)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CEBPB standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CEBPB in human glioblastoma (U87-MG) loaded at 1 mg/mL.The concentration of CEBPB was measured in duplicate, interpolated from the CEBPB standard curve, and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CEBPB concentration was determined to be 747.8 pg/mL.
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Sandwich ELISA - Human CEBPB ELISA Kit (ab233631)To learn more about the advantages of recombinant antibodies see here.