Human CD70 ELISA Kit (ab267612)
Key features and details
- Sensitivity: 0.3 ng/ml
- Range: 0.287 ng/ml - 70 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CD70 ELISA Kit
See all CD70 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
0.3 ng/ml -
Range
0.287 ng/ml - 70 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 117 93% - 145% Plasma 96 69% - 125% Cell culture media 87 68% - 123% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human CD70 ELISA Kit is designed for the quantitative determination of CD70 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human CD70 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CD70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human CD70 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human CD70 Detection Antibody 2 vials Human CD70 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD 27L
- CD 70
- CD27 L
see all -
Database links
- Entrez Gene: 970 Human
- Omim: 602840 Human
- SwissProt: P32970 Human
- Unigene: 501497 Human
- Unigene: 715224 Human
Images
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Standard curve
Human CD70 ELISA kit (ab267612) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
CD27L is an alternative name for CD70.