Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human CD44 ELISA Set (ab212086)

Key features and details

  • Sensitivity: 0.13 ng/ml
  • Range: 10 ng/ml - 0.31 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human CD44 ELISA Set
    See all CD44 kits

  • Detection method

    Colorimetric

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.13 ng/ml

  • Range

    10 ng/ml - 0.31 ng/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s CD44 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) Set is designed for the quantitative measurement of soluble CD44 in Human serum, plasma, buffered solutions and cell culture medium.


    The ELISA set contains 100 µg of unlabeled, mouse monoclonal capture antibody and 50 µg of mouse monoclonal detector antibody. Lyophilized Human CD44 protein purified from Human serum is included for quantification. The exact concentration of the protein standard can be found on the vials’ label. Detection requires an  secondary antibody labeled with biotin.


    The protocol booklet contains guidelines on how to use the ELISA Set for a sandwich ELISA with a secondary antibody for detection.


    This kit will recognize both endogenous and recombinant Human CD44.


    Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Optimization of the ELISA Set reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this ELISA Set in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.


    Additional protocol information and tip on the use of the Human CD44 ELISA Set for sandwich ELISA can be found on our website:


     https://www.abcam.com/kits/how-to-use-matched-antibody-pair-kits-for-sandwich-elisa 


    For additional information on the performance of the antibody pair used in this ELISA Set, please see our equivalent CD44 Human ELISA Set (ab45912) which uses the same antibody pair.

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components Identifier 100 tests
    Human CD44 Capture Antibody 1 x 100µg
    Biotinylated anti-CD44 anitibody Red 1 x 50µg
    Human CD44 Standard Protein 1 x 139ng

  • Research areas

  • Function

    Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

  • Tissue specificity

    Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

  • Sequence similarities

    Contains 1 Link domain.

  • Domain

    The lectin-like LINK domain is responsible for hyaluronan binding.

  • Post-translational
    modifications

    Proteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
    N-glycosylated.
    O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
    Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • LHR
    • BA-1
    • CD 44
    • CD44
    • CD44 antigen
    • CD44 molecule
    • CD44 molecule (Indian blood group)
    • CD44_HUMAN
    • CDw44
    • CDW44 antigen
    • Cell surface glycoprotein CD44
    • chondroitin sulfate proteoglycan 8
    • CSPG8
    • ECMR-III
    • Epican
    • Extracellular matrix receptor III
    • GP90 lymphocyte homing/adhesion receptor
    • HCELL
    • hematopoietic cell E- and L-selectin ligand
    • Heparan sulfate proteoglycan
    • Hermes antigen
    • homing function and Indian blood group system
    • HSA
    • HUTCH-I
    • HUTCH1
    • HUTCHI
    • Hyaluronate receptor
    • IN
    • INLU-related p80 Glycoprotein
    • MC56
    • MDU2
    • MDU3
    • MGC10468
    • MIC4
    • MUTCH I
    • MUTCH1
    • PGP-1
    • PGP-I
    • PGP1
    • Phagocytic glycoprotein 1
    • Phagocytic glycoprotein I
    • Soluble CD44
    see all

  • Database links

    Images

    • Dose Response Curve (Logarithmic)
      Dose Response Curve (Logarithmic)

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Human CD44 ELISA Set (ab212086)

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