Human Carcino Embryonic Antigen ELISA Kit (CEA) (ab99992)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.343 ng/ml - 250 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Carcino Embryonic Antigen ELISA Kit (CEA) -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
Range
0.343 ng/ml - 250 ng/mlRecovery
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 116.7 93% - 136% Serum 145.6 135% - 156% Plasma 103.8 82% - 124% Assay duration
Multiple steps standard assaySpecies reactivity
Reacts with: HumanProduct overview
Abcam’s Carcinoembryonic Antigen (CEA) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CEA in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human CEA coated on a 96-well plate. Standards and samples are pipetted into the wells and CEA present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CEA antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CEA bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human Carcinoembryonic Antigen ELISA Kit (CD66e) (ab183365) from our SimpleStep ELISA® range.
Notes
Optimization may be required with urine samples.
Platform
MicroplateProperties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CEA (lyophilized) 2 vials CEA Microplate (12 x 8 well strips) 1 unit Recombinant Human CEA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). -
Cellular localization
Cell Membrane -
Alternative names
- Carcinoembryonic antigen
- Carcinoembryonic antigen related cell adhesion molecule 5
- CD66e
see all -
Database links
- Entrez Gene: 1048 Human
- SwissProt: P06731 Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1,000X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CEA (lyophilized) 2 vials CEA Microplate (12 x 8 well strips) 1 unit Recombinant Human CEA Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
Carcino Embryonic Antigen (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). -
Cellular localization
Cell Membrane -
Alternative names
- Carcinoembryonic antigen
- Carcinoembryonic antigen related cell adhesion molecule 5
- CD66e
see all -
Database links
- Entrez Gene: 1048 Human
- SwissProt: P06731 Human
Images
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Sandwich ELISA - Human CEA (ab99992)
CEA measured in biological fluids showing quantity (ng) per mL of tested sample
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Typical Standard CurveRepresentative Standard Curve using ab99992.
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Typical Standard CurveRepresentative Standard Curve using ab99992.