Human BACE-1 ELISA Kit (ab267637)
Key features and details
- Sensitivity: 1 ng/ml
- Range: 1.024 ng/ml - 250 ng/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human BACE-1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
1 ng/ml -
Range
1.024 ng/ml - 250 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 69% - 121% Plasma 92 78% - 100% Cell culture media 98 78% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Product overview
Human BACE-1 ELISA Kit is designed for the quantitative determination of BACE-1 in cell culture supernatants, plasma and serum samples.
ΔNote: Human BACE-1 concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human BACE-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and BACE-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human BACE-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BACE-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA antibody pair detects human BACE-1, it also can recognize mouse BACE-1.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Human BACE-1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human BACE-1 Detection Antibody 2 vials Human BACE-1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
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Alternative names
- APP beta secretase
- Asp 2
- ASP2
see all -
Database links
- Entrez Gene: 23621 Human
- Entrez Gene: 23821 Mouse
- Omim: 604252 Human
- SwissProt: P56817 Human
- SwissProt: P56818 Mouse
- Unigene: 504003 Human
- Unigene: 24044 Mouse
Images
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Standard curve
Human BACE-1 ELISA kit (ab267637) Standard curve.
Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.