Human Anti-Mycoplasma pneumoniae IgA ELISA Kit (ab108754)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-Mycoplasma pneumoniae IgA ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Neg. Serum 20 6.1% Pos. Serum 24 3.5% Inter-assay Sample n Mean SD CV% Neg. Serum 12 8.1% Pos. Serum 12 3.9% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Mycoplasma pneumoniae IgA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgA class antibodies against Mycoplasma pneumoniae in Human serum and plasma.
A 96-well plate has been precoated with Mycoplasma pneumoniae antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgA conjugate is added to the wells, which binds to the immobilized Mycoplasma pneumoniae-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Mycoplasma pneumoniae IgA sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml IgA Sample Diluent white cap 1 x 100ml Mycoplasma pneumoniae (IgA) Coated Microplate (12 x 8 wells) 12 breakapart 8-well snap-off strips 1 unit Mycoplasma pneumoniae anti-IgA HRP Conjugate black cap 1 x 20ml Mycoplasma pneumoniae IgA Cut-off Control green cap 1 x 3ml Mycoplasma pneumoniae IgA Negative Control blue cap 1 x 2ml Mycoplasma pneumoniae IgA Positive Control red cap 1 x 2ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
