Human Annexin V Matched Antibody Pair Kit (ab221431)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 781.25 pg/ml - 25000 pg/ml
Overview
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Product name
Human Annexin V Matched Antibody Pair Kit
See all Annexin V/ANXA5 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
78.9 pg/ml -
Range
781.25 pg/ml - 25000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Annexin V Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Annexin V.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab223863. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Annexin V Capture Antibody 2 x 50µg 1 x 50µg Human Annexin V Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Annexin V Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
Involvement in disease
Pregnancy loss, recurrent, 3 -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsS-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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Alternative names
- Anchorin CII
- Annexin 5
- Annexin A5
see all -
Database links
- Entrez Gene: 308 Human
- Omim: 131230 Human
- SwissProt: P08758 Human
- Unigene: 480653 Human
Images
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Human Annexin V standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Annexin V Matched Antibody Pair Kit (ab221431)To learn more about the advantages of recombinant antibodies see here.
