Human AKR1B10 ELISA Kit (ab254506)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.3 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell Lysate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human AKR1B10 ELISA Kit
See all AKR1B10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 2.3% Inter-assay Sample n Mean SD CV% Extract 3 3.7% -
Sample type
Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
10.3 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell Lysate 112 106% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human AKR1B10 ELISA Kit (ab254506) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AKR1B10 protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human AKR1B10 with 10.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Aldo-Keto Reductase Family-1 member B10 (AKR1B10), also referred to as Aldose Reductase-like protein, is an 316- amino acid enzyme encoded by the AKR1B10 gene. AKR1B10 belongs to the aldo/keto reductase superfamily, where AKR1B10’s function is to catalyze the NADPH-dependent reduction of carbonyls to the corresponding alcohols. Found to be highly expressed in the small intestine and colon, AKR1B10 is thought to be responsible in detoxifying dietary and lipid derived carbonyls and may play a role in liver carcinogenesis. AKR1B10 has no counterpart in mouse or rat species.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human AKR1B10 Capture Antibody 1 x 600µl 10X Human AKR1B10 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human AKR1B10 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. -
Tissue specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. -
Sequence similarities
Belongs to the aldo/keto reductase family. -
Cellular localization
Lysosome. Secreted. Secreted through a lysosome-mediated non-classical pathway. - Information by UniProt
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Alternative names
- AK1BA_HUMAN
- AKR1B10
- AKR1B11
see all -
Database links
- Entrez Gene: 57016 Human
- Omim: 604707 Human
- SwissProt: O60218 Human
- Unigene: 116724 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human AKR1B10 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.The AKR1B10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native AKR1B10 in human A549 cell extract based on a 0.31 µg/mL extract load.The concentrations of AKR1B10 were measured in duplicate and interpolated from the AKR1B10 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Sandwich ELISA - Human AKR1B10 ELISA Kit (ab254506)To learn more about the advantages of recombinant antibodies see here.
