HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)
![HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)](https://www.abcam.com/ps/products/204/ab204456/Images/ab204456-253840-anti-hnrnp-k-antibody-f45-p9-c7-hrp-immunohistochemistry.jpg "HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)")
Key features and details
- HRP Mouse monoclonal [F45 P9 C7] to hnRNP K
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
- Isotype: IgG1
Overview
-
Product name
HRP Anti-hnRNP K antibody [F45 P9 C7]
See all hnRNP K primary antibodies -
Description
HRP Mouse monoclonal [F45 P9 C7] to hnRNP K -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Chicken
-
Immunogen
-
Positive control
- WB: HEK293, NIH3T3 and PC12 whole cell lysates. IHC-P: FFPE Human Kidney Normal
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
F45 P9 C7 -
Myeloma
Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)
IHC image of hnRNP K staining in a section of formalin-fixed paraffin-embedded Normal Human Kidney*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab204456, 1/50 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
![Western blot - HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)](https://www.abcam.com/ps/products/204/ab204456/Images/ab204456-252321-anti-hnrnp-k-antibody-f45-p9-c7-hrp-western-blot.jpg)
Western blot - HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456)All lanes : HRP Anti-hnRNP K antibody [F45 P9 C7] (ab204456) at 1/5000 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 51 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab204456 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
