HRP Anti-Fluorescein antibody (ab6656)
Key features and details
- HRP Goat polyclonal to Fluorescein
- Suitable for: ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Fluorescein antibody
See all Fluorescein primary antibodies -
Description
HRP Goat polyclonal to Fluorescein -
Host species
Goat -
Conjugation
HRP -
Specificity
This antibody reacts with FITC-conjugated proteins. -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Fluorescein conjugated to Goat IgG
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Fluorescein antibody (ab6656) This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of Mouse thymus in which the medullary epithellai cells are labelled with fluorescein-conjugated UEA1 lectin with ab6656. Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 40 minutes at 20°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 20°C.
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Immunohistochemistry (Frozen sections) - HRP Anti-Fluorescein antibody (ab6656) This image is courtesy of an anonymous Abreviewab6656 staining GFP in Mouse mammary carcinoma metastasis tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Perfusion fixed tissue with 4% paraformaldehyde, blocked with 0.5% Perkin-Elmer TNB blocking reagent for 30 minutes at 25°C and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/500) for 30 minutes at 25°C
Micrograph demonstrates use of ab6662 for detection of GFP-positive tumor cells (LEFT) and an adjacent section after amplification with ab6656 (RIGHT). Images were taken with identical settings on a Leica confocal microscope.
