Human HE4 ELISA Kit (ab240688)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 5.2 pg/ml
- Range: 23.4 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HE4 ELISA Kit
See all HE4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% supernatant 5 3.9% Inter-assay Sample n Mean SD CV% Supernatant 3 6.2% -
Sample type
Cell culture supernatant, Saliva, Urine, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.2 pg/ml -
Range
23.4 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 98% - 112% Saliva 118 111% - 123% Urine 108 106% - 111% Serum 108 99% - 116% EDTA Plasma 115 111% - 119% Cit plasma 100 88% - 115% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human HE4 ELISA Kit (ab240688) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of HE4 protein in cit plasma, edta plasma, saliva, serum, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HE4 with 5.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human HE4 Capture Antibody 1 x 600µl 10X Human HE4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml Antibody Diluent 4BI 1 x 6ml Human HE4 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis. -
Sequence similarities
Contains 2 WAP domains. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- dJ461P17.6
- EDDM4
- epididymal protein 4
see all -
Database links
- Entrez Gene: 10406 Human
- SwissProt: Q14508 Human
- Unigene: 2719 Human
Images
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Other - Human HE4 ELISA Kit (ab240688)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human HE4 Standard Curve Comparison.Standard Curve comparison between human HE4 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human HE4 standard curve in Sample Diluent 25BS + Enhancer.The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of human HE4 standard curve in Sample Diluent NS + Enhancer.The HE4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native HE4 in human serum, plasma (citrate), and plasma (EDTA) samples.The concentrations of HE4 were measured in duplicates, interpolated from the HE4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, and plasma (EDTA) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 5,723 pg/mL in serum, 5,017 pg/mL in plasma (citrate), and 5,396 pg/mL in plasma (EDTA). -
Interpolated concentrations of native HE4 in human saliva, urine, and cell culture supernatant samples.The concentrations of HE4 were measured in duplicates, interpolated from the HE4 standard curves and corrected for sample dilution. Undiluted samples are as follows: saliva 1: 1,000, urine 1: 1,000, and OVCAR-3 cell culture supernatant 1: 1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 1,405 ng/mL in saliva, 1,237 ng/mL in urine, and 530 ng/mL in OVCAR-3 cell culture supernatant. -
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HE4 concentration was determined to be 7067 pg/mL with a range of 4099 - 12,026 pg/mL.
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Sandwich ELISA - Human HE4 ELISA Kit (ab240688)To learn more about the advantages of recombinant antibodies see here.
