Anti-RAP1 antibody (ab272863)
Key features and details
- Rabbit polyclonal to RAP1
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RAP1 antibody
See all RAP1 primary antibodies -
Description
Rabbit polyclonal to RAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment within Human RAP1. The exact sequence is proprietary. Center region.
Database link: Q9NYB0 -
Positive control
- WB: HepG2 and HCT116 whole cell lysate. Mouse brain whole cell lysate. IHC-P: SCM1 xenograft tissue. ICC/IF: A-375 cells. IP: HepG2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RAP1 antibody (ab272863)All lanes : Anti-RAP1 antibody (ab272863) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAP1 antibody (ab272863)Paraffin-embedded SCM1 xenograft tissue stained for RAP1 using ab272863 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Immunocytochemistry/ Immunofluorescence - Anti-RAP1 antibody (ab272863)4% PFA-fixed A-375 (human malignant melanoma cell line) cells stained for RAP1 using ab272863 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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Immunoprecipitation - Anti-RAP1 antibody (ab272863)IP Sample: HepG2 whole cell lysate/extract.
10% SDS-PAGE
All lanes : Anti-RAP1 antibody (ab272863) at 1/1000 dilution
Lane 1 : Control with 3 µg of pre-immune rabbit IgG
Lane 2 : Immunoprecipitation of RAP1 by 3 µg of ab272863
Secondary
All lanes : anti-rabbit IgG (HRP)
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Western blot - Anti-RAP1 antibody (ab272863)Anti-RAP1 antibody (ab272863) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg10% SDS PAGE