HRP Anti-CD31 antibody [JC/70A] (ab215774)
![HRP Anti-CD31 antibody [JC/70A] (ab215774)](https://www.abcam.com/ps/products/215/ab215774/Images/ab215774-291657-anti-cd31-antibody-jc-70a-hrp-immunohistochemistry.jpg "HRP Anti-CD31 antibody [JC/70A] (ab215774)")
Key features and details
- HRP Mouse monoclonal [JC/70A] to CD31
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: HRP
- Isotype: IgG1
Overview
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Product name
HRP Anti-CD31 antibody [JC/70A]
See all CD31 primary antibodies -
Description
HRP Mouse monoclonal [JC/70A] to CD31 -
Host species
Mouse -
Conjugation
HRP -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Tissue, cells or virus corresponding to Human CD31. (Membrane preparation of a spleen from a patient with hairy cell leukaemia).
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Positive control
- IHC-P: normal human tonsil tissue sections
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
JC/70A -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-CD31 antibody [JC/70A] (ab215774)
IHC image of CD31 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab215774, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
