Anti-CD63 antibody [MX-49.129.5] (ab193349)
![Anti-CD63 antibody [MX-49.129.5] (ab193349)](https://www.abcam.com/ps/products/193/ab193349/Images/ab193349-243037-anti-cd63-antibody-mx-491295-immunohistochemistry.jpg "Anti-CD63 antibody [MX-49.129.5] (ab193349)")
Key features and details
- Mouse monoclonal [MX-49.129.5] to CD63
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD63 antibody [MX-49.129.5]
See all CD63 primary antibodies -
Description
Mouse monoclonal [MX-49.129.5] to CD63 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Full length protein corresponding to Human CD63 aa 1 to the C-terminus.
Database link: P08962 -
Positive control
- SK-MEL-28, HL60 or THP-1 cells. Melanoma or lymphoma; Flow Cyt: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MX-49.129.5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [MX-49.129.5] (ab193349)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue labeling CD63 with ab193349. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The antibody was applied at 0.5 ug/ml for 30 minutes at room temperature.
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![Flow Cytometry - Anti-CD63 antibody [MX-49.129.5] (ab193349)](https://www.abcam.com/ps/products/193/ab193349/Images/ab193349-6-anti-cd63-antibody-flowcytometry-mcf-7-cell.jpg)
Flow Cytometry - Anti-CD63 antibody [MX-49.129.5] (ab193349)Flow cytometry of MCF-7 cells unstained (gray) or stained with ab193349 (orange). -
![Western blot - Anti-CD63 antibody [MX-49.129.5] (ab193349)](https://www.abcam.com/ps/products/193/ab193349/Images/ab193349-243035-anti-cd63-antibody-mx-491295-western-blot.jpg)
Western blot - Anti-CD63 antibody [MX-49.129.5] (ab193349)Anti-CD63 antibody [MX-49.129.5] (ab193349) at 0.5 µg/ml + Human Spleen at 2 µg
Predicted band size: 26 kDa
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![Flow Cytometry - Anti-CD63 antibody [MX-49.129.5] (ab193349)](https://www.abcam.com/ps/products/193/ab193349/Images/ab193349-5-anti-cd63-antibody-flowcytometry-mcf-7-cells.jpg)
Flow Cytometry - Anti-CD63 antibody [MX-49.129.5] (ab193349)Flow cytometry of MCF-7 cells unstained (gray) or stained with ab193349 (green).
