Histone H4 Acetylation Assay Kit (ab115103)
Key features and details
- Assay type: Cell-based (quantitative)
- Platform: Microplate reader
- Assay time: 5 hr
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Histone H4 Acetylation Assay Kit
See all Histone H4 acetylation kits -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Cell-based (quantitative) -
Assay time
5h 00m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Acetylation of histones such histone H4 has been involved in the regulation of chromatin structure and the recruitment of transcription factors to gene promoters. HATs (histone acetyltransferases) and HDACs (histone deacetylases) play a critical role in controlling histone H4 actylation. Histone acetylation is tightly involved in cell cycle regulation, cell proliferation and apoptosis. Reversible acetylation of nucleosomal histone H4 is believed to correlate with potential transcriptionally activity of eukaryotic chromatin domains.
Histone H4 Acetylation Assay Kit (ab115103) allows the user to measure global histone H4 acetylation at tremendously fast speeds and consistency, superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment. The kit is suitable for specifically measuring global histone H4 acetylation using a variety of mammalian cells including fresh and frozen tissues, and cultured adherent and suspension cells.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Lysis Buffer 1 x 5ml 1 x 10ml 10X Wash Buffer 1 x 14ml 1 x 28ml 8-Well Assay Strips (with Frame) 6 units 12 units Acetylated Histone H4 Control (60 µg/mL) 1 x 10µl 1 x 20µl Antibody Buffer 1 x 6ml 1 x 12ml Blocking Buffer 1 x 10ml 1 x 20ml Capture Antibody, 100 µg/mL 1 x 25µl 1 x 50µl Detection Antibody, 400 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 5ml 1 x 10ml Extraction Buffer 1 x 8ml 1 x 16ml Histone Buffer 1 x 0.5ml 1 x 1ml Stop Solution 1 x 3ml 1 x 6ml -
Research areas