Histone H3 Total Quantification Kit (Fluorometric) (ab115092)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 2 ng/well
Overview
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Product name
Histone H3 Total Quantification Kit (Fluorometric)
See all Histone H3 kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Sensitivity
2 ng/well -
Range
10 ng/well - 1000 ng/well -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Mammals
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Product overview
Histone H3 can undergo several epigenetic modifications that influence cellular processes such as transcription activation/inactivation, chromosome packaging, and DNA damage/repair. These modifications including acetylation, phosphorylation or methylation occur on the N-terminal tail domains of histone H3 through a variety of histone modifying enzymes. In most species, histone H3 is primarily acetylated at K9, K14, K18, K23 and K56, methylated at K4, K9, K27, K36 and K79, and phosphorylated at S10, S28, T3 and T11, respectively.
Histone H3 Total Quantification Kit (Fluorometric) (ab115092) enables the user to quantify levels of histone H3 proteins independent of their modified states. It can also be used for normalizing the modified histone H3 content of samples when run in parallel with Abcam's histone modification quantification kit series. ab115091 is suitable for specifically measuring total histone H3 on mammalian samples such as human, mouse, and rat, including fresh and frozen tissues and cultured adherent and suspension cells.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8 well Sample Strips (with frame) 4 units 9 units 8 well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Fluoro Developer 1 x 12µl 1 x 24µl Fluoro Dilution 1 x 4ml 1 x 8ml Fluoro Enhancer 1 x 12µl 1 x 24µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl -
Research areas
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Cellular localization
Nucleus. Chromosome. -
Alternative names
- H3f3a
- H3FA
- H3FB
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Database links
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
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Images
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Functional Studies - Histone H3 Quantification Assay Kit (ab115092)
A histone H3 standard curve was generated as described in the protocol of the Histone H3 Total Quantification Kit (Fluorometric) (ab115092).
