Anti-ICAD antibody (ab264202)
Key features and details
- Rabbit polyclonal to ICAD
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ICAD antibody
See all ICAD primary antibodies -
Description
Rabbit polyclonal to ICAD -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human ICAD aa 271-331. The exact sequence is proprietary.
Database link: O00273 -
Positive control
- IHC-P: Human ovarian cancer tissue; WB: HeLa and HEK-293T whole cell lysates; IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ICAD antibody (ab264202)All lanes : Anti-ICAD antibody (ab264202) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µgDetected with chemilimuniscence with exposure time of 30 seconds.
4-20% SDS-PAGE.
Secondary: Goat anti-Rabbit Light Chain HRP Conjugate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICAD antibody (ab264202)Immunohistochemical analysis of formalin/PFA-fixed, paraffin-embedded human ovarian tissue sections labeling ICAD with ab264202 at 1/200 dilution. Detection: DAB.
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Immunoprecipitation - Anti-ICAD antibody (ab264202)ICAD was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264202 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264202 at 1 µg/ml.
Lane 1: ab264202 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 1 sec.
