Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.5 ng/well
Overview
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Product name
Histone H3 Modification Multiplex Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
0.5 ng/well -
Range
20 ng/well - 500 ng/well -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
The Histone H3 Modification Multiplex Assay Kit (Colorimetric) (ab185910) is a complete set of optimized reagents to detect and quantify up to 21 modified Histone H3 patterns simultaneously in a simple, ELISA-like format with use of a standard microplate reader.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 96 tests 10X Wash Buffer 1 x 28ml 8-Well Strips 2 units 96-Well Strip Plate (with Frame) 1 unit Adhesive Covering Film 1 unit Antibody Buffer 1 x 8ml Assay Control Protein 1 x 20µl Detection Antibody 1 x 12µl Developer Solution 1 x 12ml Stop Solution 1 x 12ml -
Research areas
Images
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Typical data
Histone extracts were prepared from MCF-7 and MDA-231 cells and multiple Histone H3 modifications were screened and measured using the Histone H3 Modification Multiplex Assay Kit (Colorimetric). 100 ng of total histone proteins were used.
