Heme Assay Kit (ab272534)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Plasma, Serum, Urine, Whole Blood
Overview
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Product name
Heme Assay Kit -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Whole Blood -
Assay type
Quantitative -
Range
0.6 µM - 125 µM -
Product overview
Heme Assay Kit (ab272534) is a simple, direct and automation-ready procedure for measuring heme concentration. This Assay Kit is based on an improved aqueous alkaline solution method, in which the heme is converted into a uniform colored form. The intensity of color, measured at 400 nm, is directly proportional to the heme concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Sensitive and accurate: Linear detection range 0.6 – 125 μM heme in 96-well plate assay.
Simple and high-throughput: The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
Safety: Reagents are non-toxic.
Versatility: Assays can be executed in 96-well plate or cuvette.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 250 tests Calibrator 1 x 10ml Reagent 1 x 50ml
Images
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Heme Assay Kit standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
