HA-1077 (Fasudil), ROCK2 inhibitor (ab120306)
Key features and details
- ROCK2 inhibitor
- CAS Number: 103745-39-7
- Purity: > 98%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
HA-1077 (Fasudil), ROCK2 inhibitor -
Description
ROCK2 inhibitor -
Alternative names
- Fasudil
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Biological description
ROCK2 inhibitor (IC50 = 1.9 µM). Also shown to inhibit PRK2 (IC50 = 4 µM), MSK1 (IC50 = 5 µM), Rsk2 (IC50 = 15 µM), PKA, p70 S6 Kinase, CaM Kinase and MLCK. Calcium antagonist and vasodilator.
Also available in simple stock solutions (ab146700) - add 1 ml of water to get an exact, ready-to-use concentration. -
Purity
> 98% -
CAS Number
103745-39-7 -
Chemical structure
Properties
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Chemical name
1-(5-Isoquinolinylsulfonyl)homopiperazine hydrochloride -
Molecular weight
327.83 -
Molecular formula
C14H17N3O2S.HCl -
PubChem identifier
163751 -
Storage instructions
Store at Room Temperature. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 100 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cl.O=S(=O)(c2cccc1cnccc12)N3CCCNCC3 -
Source
Synthetic
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Research areas
Images
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Functional Studies - HA-1077 (Fasudil), ROCK2 inhibitor (ab120306)ab46154 staining VEGF in MCF7 cells treated with HA 1077 (ab120306), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of HA 1077 as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120306 (HA 1077) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab46154 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
