Fluorescein 5-maleimide, Fluorescent protein labeling reagent (ab145102)
Key features and details
- Fluorescent protein labeling reagent.
- CAS Number: 75350-46-8
- DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Fluorescein 5-maleimide, Fluorescent protein labeling reagent -
Description
Fluorescent protein labeling reagent. -
Alternative names
- 1-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione
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Biological description
Activated fluorescent molecule used to conjugate fluorescein to proteins. The maleimide group reacts optimally with sulfhydryl groups on cysteine side chains at pH 7, forming a stable thioether bond.
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CAS Number
75350-46-8 -
Chemical structure
Properties
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Excitation
494nm -
Emission
519nm -
Chemical name
1-(3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-1H-pyrrole-2,5-dione -
Molecular weight
427.36 -
Molecular formula
C24H13NO7 -
Storage instructions
Store at -20°C. Store In the Dark. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes. -
Solubility overview
DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic