FITC Anti-CD8 alpha antibody [3-298] (ab24910)
![FITC Anti-CD8 alpha antibody [3-298] (ab24910)](https://www.abcam.com/ps/products/24/ab24910/Images/ab24910-1-anti-cd8-alpha-antibody-3-298-fitc-flow-cytometry-peripheral-blood-lymphocytes-chicken.jpg "FITC Anti-CD8 alpha antibody [3-298] (ab24910)")
Key features and details
- FITC Mouse monoclonal [3-298] to CD8 alpha
- Suitable for: Flow Cyt
- Reacts with: Chicken
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-CD8 alpha antibody [3-298]
See all CD8 alpha primary antibodies -
Description
FITC Mouse monoclonal [3-298] to CD8 alpha -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
This antibody recognises CD8a. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Chicken
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Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Flow Cyt: Chicken peripheral blood lymphocytes.
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General notes
In chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Primary antibody notes
In chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, butClonality
MonoclonalClone number
3-298Isotype
IgG2bLight chain type
kappaResearch areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species Flow Cyt ChickenAll applications TurkeyApplication Abreviews Notes Flow Cyt Use at an assay dependent concentration.Notes Flow Cyt
Use at an assay dependent concentration.Target
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Function
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
Involvement in disease
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsAll of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
Images
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Flow Cytometry - Anti-CD8 alpha antibody [3-298] (FITC) (ab24910)
Chicken peripheral blood lymphocytes were costained using CD8 alpha-FITC and CD4-PE to distinguish cell subpopulations. Cells were incubated with anti-CD8 alpha antibody [3-298] (FITC) (ab24910) at 1 µg/106 cells.
References (0)
ab24910 has not yet been referenced specifically in any publications.
Images
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Flow Cytometry - Anti-CD8 alpha antibody [3-298] (FITC) (ab24910)
Chicken peripheral blood lymphocytes were costained using CD8 alpha-FITC and CD4-PE to distinguish cell subpopulations. Cells were incubated with anti-CD8 alpha antibody [3-298] (FITC) (ab24910) at 1 µg/106 cells.
