Astana Biomed Group, an authorized Abcam distributor in Central Asia

Feline PDGF-BB ELISA Kit (ab273234)

Key features and details

  • Sensitivity: 5.4 pg/ml
  • Range: 5.49 pg/ml - 4000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Cat

Overview

  • Product name

    Feline PDGF-BB ELISA Kit
    See all PDGF B kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    5.4 pg/ml

  • Range

    5.49 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 138.9 128% - 148%
    Plasma 133.7 126% - 148%
    Cell culture media 107.7 92% - 117%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Cat

  • Product overview

    Feline PDGF-BB ELISA Kit (ab273234) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Feline PDGF-BB in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for feline PDGF-BB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-feline PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Example data
    Example data

    This standard curve is for demonstration only. A standard curve must be run with each assay.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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