Factor XIa Assay Kit (ab241037)
Key features and details
- Detection method: Colorimetric
Overview
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Product name
Factor XIa Assay Kit -
Detection method
Colorimetric -
Product overview
The Factor XIa Assay Kit (ab241037) utilizes the ability of factor XIa to cleave a synthetic substrate to release p-Nitroaniline (pNA) which can be quantitatively measured by a colorimetric assay (OD 405 nm).
The kit is easy-to-use and can detect Factor XIa (as low as 1 mPEU) from plasma and purified protein samples.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests FXIa Activator 1 x 1ml FXIa Assay Buffer 1 x 25ml FXIa Substrate 1 x 1ml Human Factor XIa 1 vial Inhibitor 1 1 x 0.1ml Inhibitor 2 1 x 0.1ml pNA Standard (0.1 M) 1 x 20µl -
Research areas
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Function
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. -
Tissue specificity
Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells. -
Involvement in disease
Defects in F11 are the cause of factor XI deficiency (FA11D) [MIM:612416]; also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsActivated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- coagulation factor XI
- Coagulation factor XIa light chain
- F11
see all
Images
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Kinetic progressive curves for different amounts of FXIa Enzyme.Kinetic progressive curves for different amounts of FXIa Enzyme.
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Kinetic progressive curves for different amounts Activated Plasma Samples.Kinetic progressive curves for different amounts Activated Plasma Samples. -
Standard curve for pNA.Standard curve for pNA. -
FXIa activity in plasma.Standard curve for pNA (n = 3) was used to estimate FXIa activity in plasma (n = 5). Assays were performed according to the kit protocol.