Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16618-90] to Macrophage Inflammatory Protein 1 alpha / CCL3 - BSA and Azide free
  • Suitable for: Sandwich ELISA, Flow Cyt, IP, ICC/IF, EIA, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free
    See all Macrophage Inflammatory Protein 1 alpha / CCL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR16618-90] to Macrophage Inflammatory Protein 1 alpha / CCL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISA, Flow Cyt, IP, ICC/IF, EIA, WBmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: RAW 264.7 cells treated with 100 ng/ml Lipopolysaccharide for 3h, followed by 300 ng/ml Brefeldin A for 3h.

  • General notes

    Ab229699 is the carrier-free version of ab179638. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229699 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)
    Immunoprecipitation - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

    Macrophage Inflammatory Protein 1 alpha / CCL3 was immunoprecipitated from 0.35 mg RAW 264.7 (mouse  macrophage cell line transformed with Abelson murine leukemia virus), treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours, whole cell lysate with ab179638 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab179638 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate 10 µg (Input).

    Lane 2: ab179638 IP in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179638 in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    High sensitivity ECL substrate used to develop the blot.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

  • Flow Cytometry - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)
    Flow Cytometry - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (mouse  macrophage cell line transformed with Abelson murine leukemia virus) cell line, treated with 100 ng/ml Lipopolysaccharide for 3 hours, then add 300 ng/ml Brefeldin A for the last 3 hours (red) / Untreated control (green), labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab179638 at 1/600 compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

  • Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)
    Immunocytochemistry/ Immunofluorescence - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) untreated or treated with 100 ng/ml Lipopolysaccharide for 3 hours, followed by 300 ng/ml Brefeldin A for 3 hours cells labeling Macrophage Inflammatory Protein 1 alpha / CCL3 with ab179638 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing positive staining in RAW 264.7 treated with 100 ng/ml Lipopolysaccharide for 3 hours, followed by 300 ng/ml Brefeldin A for 3 hours.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

     

  • Sandwich ELISA - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)
    Sandwich ELISA - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

    Standard Curve for Macrophage Inflammatory Protein 1 alpha / CCL3 (Analyte: Recombinant mouse Macrophage Inflammatory Protein 1 alpha / CCL3 protein) dilution range 0-5 ng/mL using Capture antibody at 0.2 ug/mL and Detector Antibody at 0.5 ug/mL. Secondary antibody: Peroxidase Streptavidin SA-HRP at 1/20000 dilution. Concentration of ab179638 may vary from lot to lot; please use this curve as guideline.

    Washing buffer: 1X PBST
    Diluting/Blocking buffer and concentration: 1% BSA/PBS

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab179638).

     

  • Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)
    Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody [EPR16618-90] - BSA and Azide free (ab229699)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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