Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric) (ab273322)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.1 mU/well
Overview
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Product name
Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric)
See all ethanolamine kinase kits -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.1 mU/well -
Assay duration
Multiple steps standard assay -
Product overview
Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric) (ab273322) is suitable for detecting ETNK enzyme activity in different tissues and it can detect as low as 0.1 mU of ETNK in biological samples. Samples include cell culture, crude lysate and animal tissues (heart, liver, kidney, lung, and muscle, etc.).
In the assay ENTK phosphorylates Ethanolamine producing Phosphoethanolamine and ADP. The produced ADP is then detected with a set of enzymatic reactions that generate fluorescent product (Ex/Em: 535/587 nm). The fluorescence signal is directly proportional to the generated ADP.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ADP Standard (1 µmol) 1 vial ENTK Assay Buffer 1 x 25ml ENTK Converter (Lyophilized) 1 vial ENTK Developer (Lyophilized) 1 vial ENTK Probe 1 x 0.2ml ENTK Substrate 1 vial ETNK Enzyme 1 x 20µl -
Research areas
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Function
Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis. -
Tissue specificity
Expressed in kidney, liver, placenta, heart, leukocyte, ovary and testis. -
Pathway
Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3. -
Sequence similarities
Belongs to the choline/ethanolamine kinase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- 1110061E11Rik
- 4930555L11Rik
- AI195356
see all
Images
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ADP Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
ADP Standard Curve.
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Reaction curvesExample data.
Reaction curves of ETNK vs. no enzyme control in the assay.
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Specific activity of ETNK2Example data.
Specific ETNK2 activity in different mouse tissues determined using the kit protocol.
