Astana Biomed Group, an authorized Abcam distributor in Central Asia

Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric) (ab273322)

Key features and details

  • Assay type: Enzyme activity (quantitative)
  • Detection method: Fluorescent
  • Platform: Microplate
  • Sample type: Adherent cells, Suspension cells, Tissue
  • Sensitivity: 0.1 mU/well

Overview

  • Product name

    Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric)
    See all ethanolamine kinase kits

  • Detection method

    Fluorescent

  • Sample type

    Tissue, Adherent cells, Suspension cells

  • Assay type

    Enzyme activity (quantitative)

  • Sensitivity

    0.1 mU/well

  • Assay duration

    Multiple steps standard assay

  • Product overview

    Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric) (ab273322) is suitable for detecting ETNK enzyme activity in different tissues and it can detect as low as 0.1 mU of ETNK in biological samples. Samples include cell culture, crude lysate and animal tissues (heart, liver, kidney, lung, and muscle, etc.).


    In the assay ENTK phosphorylates Ethanolamine producing Phosphoethanolamine and ADP. The produced ADP is then detected with a set of enzymatic reactions that generate fluorescent product (Ex/Em: 535/587 nm). The fluorescence signal is directly proportional to the generated ADP.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    ADP Standard (1 µmol) 1 vial
    ENTK Assay Buffer 1 x 25ml
    ENTK Converter (Lyophilized) 1 vial
    ENTK Developer (Lyophilized) 1 vial
    ENTK Probe 1 x 0.2ml
    ENTK Substrate 1 vial
    ETNK Enzyme 1 x 20µl

  • Research areas

  • Function

    Highly specific for ethanolamine phosphorylation. May be a rate-controlling step in phosphatidylethanolamine biosynthesis.

  • Tissue specificity

    Expressed in kidney, liver, placenta, heart, leukocyte, ovary and testis.

  • Pathway

    Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

  • Sequence similarities

    Belongs to the choline/ethanolamine kinase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Alternative names

    • 1110061E11Rik
    • 4930555L11Rik
    • AI195356
    • AI841245
    • C80956
    • D6Ertd3e
    • EKI
    • EKI 1
    • EKI1
    • EKI1_HUMAN
    • Ethanolamine kinase 1
    • ETNK1
    • Nbla10396
    • Putative protein product of Nbla10396
    see all

Images

  • ADP Standard Curve
    ADP Standard Curve

    Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

    ADP Standard Curve.

  • Reaction curves
    Reaction curves

    Example data.

    Reaction curves of ETNK vs. no enzyme control in the assay.

  • Specific activity of ETNK2
    Specific activity of ETNK2

    Example data.

    Specific ETNK2 activity in different mouse tissues determined using the kit protocol.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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