ab12793 RNA polymerase II CTD repeat YSPTSPS peptide - phospho S2 0.1 µg/ml was mixed with GST tagged recombinant proteins in vitro in binding buffer for 1 hour at RT. Glutathione coated magnetic beads were then mixed into the buffer and removed after another hour incubation. The GST tagged recombinant proteins and any bound peptide was eluted by incubating with free glutathione. The peptides analyzed by dotblot using primary antibody ab5095(1/3000) and an undiluted conjugated HRP-Goat anti-rabbit secondary antibody.
This experiment was conducted with ab12795, ab18488 and an another peptide as a negative control.

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This peptide was used as a positive control for ab5095 (anti S2p-CTD)
Sample: Human Recombinant protein (ab12793 peptide)
Loading amount: 0.25 µg
Specification: ab12793 peptide
Gel Running Conditions: Reduced Denaturing (16% tris-tricine (Schagger et al 2006))
Blocking step: BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C

Other product details
Incubation time: 1 hour(s) and 0 minute(s) · Temperature: 23°C

Secondary antibody: HRP conjugated Goat anti-rabbit polyclonal, at a 1/20,000 dilution.