DyRect™ Live-Cell Neutral Lipid Imaging Kit (ab253395)
Overview
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Product name
DyRect™ Live-Cell Neutral Lipid Imaging Kit -
Product overview
This easy to use kit fluorescently labels both neutral and polar lipids in live cells. When stained with this imaging kit, neutral lipids will fluoresce green while polar lipids like phospholipids will fluoresce red. Any free dye not associated with lipid remains non-fluorescent. Can by used with flow cytometry or fluorescence microscopy.
Foam cells and lipid engorged macrophages have been found to be prime suspects in the progression of various disease states, from Alzheimer’s to atherosclerosis. They are characterized by the accumulation of intracellular neutral lipid droplets from ingesting excess lipoproteins, giving them a foam-like appearance. The intracellular fat vacuoles contain neutral lipids like cholesterol, lipoproteins and triglycerides. The DyRect Live-Cell Neutral Lipid Imaging Kit can be used to label and analyze cellular lipids. When stained with our imaging kit, neutral lipids will fluoresce green (EX 485 nm/EM 525 nm) while polar lipids like phospholipids will fluoresce red (EX552 nm/EM636 nm). Any free dye not associated with lipid remains non-fluorescent. The environmentally sensitive probe used in this staining method can be added directly to live cell cultures to label the fat vacuoles within the cells and can subsequently be analyzed by flow cytometry or fluorescence microscopy.
This kit is sold by Marker Gene under product code M2675.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 units DMSO 1 x 100µl Fluorescent Reagent 5 x 10µg Opti-Klear™ Live Cell Imaging Buffer 1X 2 x 30ml