Anti-TIP49A antibody [2943C1a] (ab51500)
![Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.com/ps/products/51/ab51500/Images/ab51500-63450-anti-tip49a-antibody-2943c1a-western-blot.jpg "Anti-TIP49A antibody [2943C1a] (ab51500)")
Key features and details
- Mouse monoclonal [2943C1a] to TIP49A
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-TIP49A antibody [2943C1a]
See all TIP49A primary antibodies -
Description
Mouse monoclonal [2943C1a] to TIP49A -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant fragment, internal sequence. (Human)
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Positive control
- HT-1080 whole cell lysate, HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.1312% Sodium phosphate, 1.05% Potassium chloride, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab51500 purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
2943C1a -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution + HT-1080 whole cell lysate at 50 µg
Secondary
Anti Mouse IgG at 1/2500 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
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![Flow Cytometry - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.com/ps/products/51/ab51500/Images/ab51500-63449-anti-tip49a-antibody-2943c1a-flow-cytometry.jpg)
Flow Cytometry - Anti-TIP49A antibody [2943C1a] (ab51500)Flow cytometry using ab51500 at 0.5ug using HeLa cells. Secondary used was an AlexaFluor® 488 conjugated Goat Anti-mouse IgG (0.25 µg). White peak shows an isotype control, while the red peak shows ab51500. -
![Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.com/ps/products/51/ab51500/Images/ab51500-63448-anti-tip49a-antibody-2943c1a-western-blot.jpg)
Western blot - Anti-TIP49A antibody [2943C1a] (ab51500) This image is courtesy of an anonymous AbreviewAll lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1 µg/ml (in 3% BSA + PBS-Tween 0.1% for 12 hours at 4°C)
Lanes 1-2 : HeLa cell lysate + control siRNA sequence
Lane 3 : HeLa cell lysate suppressed by siRNA (1) sequence to pontin
Lane 4 : HeLa cell lysate suppressed by siRNA (2) sequence to pontin
Lane 5 : Cell lysate of 293T cells
Lane 6 : Cell lysate of HeLa cells
Lane 7 : Cell lysate of U251 cells
Lane 8 : Cell lysate of Jurkat cells
Lane 9 : Cell lysate of U2OS cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-mouse IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 3% BSA for 1 hour at 21°C -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.com/ps/products/51/ab51500/Images/ab51500-63447-anti-tip49a-antibody-2943c1a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIP49A antibody [2943C1a] (ab51500)IHC image of ab51500 staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab51500, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Western blot - Anti-TIP49A antibody [2943C1a] (ab51500)](https://www.abcam.com/ps/products/51/ab51500/Images/ab51500-63446-anti-tip49a-antibody-2943c1a-western-blot.jpg)
Western blot - Anti-TIP49A antibody [2943C1a] (ab51500) Image courtesy of an anonymous Abreview.All lanes : Anti-TIP49A antibody [2943C1a] (ab51500) at 1/100 dilution
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC-12 (Rat adrenal gland pheochromocytoma) cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-mouse polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
Excellent antibody. Detects a single species of the predicted mobility in a range of common rat cell lines.
