Anti-PD-L1 antibody [EPR23546-160] (ab252436)
![Anti-PD-L1 antibody [EPR23546-160] (ab252436)](https://www.abcam.com/ps/products/252/ab252436/Images/ab252436-4-anti-pdl1-antibody-epr23546160-flow-cytometry-mouse-splenocytes.jpg "Anti-PD-L1 antibody [EPR23546-160] (ab252436)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23546-160] to PD-L1
- Suitable for: ICC, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-PD-L1 antibody [EPR23546-160]
See all PD-L1 primary antibodies -
Description
Rabbit monoclonal [EPR23546-160] to PD-L1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseICC Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow cyt: Mouse splenocytes and A20 cells. ICC: A20 and RAW 264.7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23546-160 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)
Flow cytometric analysis of Mouse splenocyte cells labelling PD-L1 with ab252436 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable MHC II (I-A/I-E) positive cells.PD-L1 is highly expressed on DC cells as described in literature (PMID: 22902234).
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![Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)](https://www.abcam.com/ps/products/252/ab252436/Images/ab252436-3-anti-pdl1-antibody-epr23546160-flow-cytometry-a20.jpg)
Flow Cytometry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)Flow cytometric analysis of A20 (Mouse reticulum sarcoma B lymphocyte) cells labelling PD-L1 with ab252436 at 1/500 dilution (0.1ug) (Red), compared with a Rabbit monoclonal IgG (ab172730) isotype contro l(Black), and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)](https://www.abcam.com/ps/products/252/ab252436/Images/ab252436-2-anti-pdl1-antibody-epr23546160-immunocytochemistry-a20.jpg)
Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 cells labelling PD-L1 with ab252436 at 1/50 (10.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in A20 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)](https://www.abcam.com/ps/products/252/ab252436/Images/ab252436-1-anti-pdl1-antibody-epr23546160-immunocytochemistry-raw-2647.jpg)
Immunocytochemistry - Anti-PD-L1 antibody [EPR23546-160] (ab252436)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 cells labelling PD-L1 with ab252436 at 1/50 (10.42 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in RAW 264.7 cell line after treatment with IFN-γ (100 ng/ml) for 24h. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Anti-PD-L1 antibody [EPR23546-160] (ab252436)](https://www.abcam.com/ps/products/252/ab252436/Images/ab252436-5-benefits-of-recombinant-antibodies.png)
Anti-PD-L1 antibody [EPR23546-160] (ab252436)
