DL-Serine Assay Kit (ab241027)
Key features and details
- Detection method: Fluorescent
- Sample type: Cell culture extracts, Cell Lysate, Cerebral Spinal Fluid, Plasma, Saliva, Serum, Tissue Homogenate, Urine
- Sensitivity: 1 µM
Overview
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Product name
DL-Serine Assay Kit -
Detection method
Fluorescent -
Sample type
Saliva, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate, Tissue Homogenate, Cerebral Spinal Fluid -
Sensitivity
Product overview
DL-Serine Assay Kit (ab241027) allows for quantification of both L- and D-Serine in biological fluids and tissues. The assay is based on the conversion of L-Serine to D-Serine, which is metabolized to an intermediate product that is subsequently oxidized and reacts with a probe to form a stable fluorophore (Ex/Em = 535/587 nm). Samples may be divided and assayed simultaneously for quantification of both D-Serine and total DL-Serine. The assay is not affected by physiological concentrations of other amino acids, is high-throughput adaptable and can detect less than 1 μM Serine.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Enzyme Mix 1 vial D-Serine Enzyme Mix 1 vial D-Serine Standard 1 vial Probe Solution 1 x 200µl Sample Cleanup Mix 1 vial Serine Assay Buffer 1 x 25µl Serine Racemase Enzyme Mix 1 vial -
Research areas
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Cellular localization
D Serine: Ubiquitous
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Developer Enzyme Mix 1 vial D-Serine Enzyme Mix 1 vial D-Serine Standard 1 vial Probe Solution 1 x 200µl Sample Cleanup Mix 1 vial Serine Assay Buffer 1 x 25µl Serine Racemase Enzyme Mix 1 vial -
Research areas
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Cellular localization
D Serine: Ubiquitous
Images
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D-Serine Standard curve.D-Serine Standard curve.
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Specificity for detection of L- or D-Serine (SER) over other amino acids.Specificity for detection of L- or D-Serine (SER) over other amino acids. At a 5-fold molar excess (10 nmoles) versus SER isomers (each 2 nmoles), L-threonine (THR) contributes ≤15% interference, L-cysteine (CYS) contributes ≤10% interference and all other amino acids contribute ≤5%.
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Estimation of total, D- and L-Serine in pooled normal human serum and CSF (10 µL).Estimation of total, D- and L-Serine in pooled normal human serum and CSF (10 µL). L-Serine concentrations for serum and CSF samples were 137.4 ± 9.06 µM and 56.01 ± 2.93 µM, whereas D-Serine concentrations were 2.37 ± 0.36 µM and 3.46 ± 0.21 µM, respectively. Data are mean ± SEM of 3 replicates, assayed according to the kit protocol.