DAPI Staining Solution (ab228549)
Overview
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Product name
DAPI Staining Solution -
Sample type
Adherent cells, Suspension cells -
Product overview
DAPI Staining Solution (ab228549) is a fluorescent stain for labeling DNA in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells and fixed cells.
Molecular Weight: 350.25
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Notes
DAPI’s absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. Although is not as strongly fluorescent as when bound to DNA, it also binds to RNA, shifting its emission to around 500 nm.
DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like FITC and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red.
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Platform
Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 2 ml DAPI (10 mM solution in water) 1 x 2ml
Images
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Chemical structure of DAPI.Chemical structure of DAPI.
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Spectrum of DAPI.Spectrum of DAPI.
