Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets) (ab133995)
Overview
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Product name
Cytokine Array – Mouse Cytokine Antibody Array (Membrane, 62 Targets)
See all Cytokine Antibody Array antibody arrays -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Whole Blood, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Semi-quantitative -
Species reactivity
Reacts with: Mouse -
Product overview
ab133995 is for simultaneous detection of 62 Mouse Cytokines. Suitable for all sample types.
Targets: Axl, BLC, CD30 L, CD30, CD40, CRG-2, CTACK, CXCL16, Eotaxin, Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CSF, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1beta, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, IL-1alpha, IL-2, IL-3, IL-3 Rbeta, IL-4, IL-5, IL-6, IL-9, KC/CXCL1, Leptin/OB, Leptin R, LIX, L-Selectin, Lymphotactin, MCP1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3beta, MIP-3alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1alpha, sTNF RI, sTNF RII, TARC, TCA-3, TECK, TIMP-1, TNFalpha, Thrombopoietin, VCAM-1, VEGF-A
Cytokine arrays are an antibody-pair-based assay, analogous to ELISA, but using a membrane as a substrate rather than a plate. Capture antibodies are supplied arrayed/spotted on a membrane with each pair of spots representing a different analyte. Sample is added (0.2-1ml of 1 sample to each membrane), and then paired biotinylated detector antibodies and streptavidin HRP. The cytokine array is analyzed using the same methods as a chemiluminescent western blot. Comparison between samples can be by eye or using densitometry software for a semi-quantitative comparison.
Learn more about membrane antibody arrays
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Notes
If you are interested in this cytokine array, a table listing all of our mouse membrane antibody cytokine arrays and other arrays and the analytes they measure is available here.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Multiplex Protein Detectionmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 4 Membranes 1 x 8 Membranes 1 x 2 Membranes 1,000X HRP-Conjugated Streptavidin 1 x 50µl 1 x 50µl 1 x 50µl 1X Blocking Buffer 1 x 25ml 2 x 25ml 1 x 25ml 20X Wash Buffer I 1 x 10ml 1 x 20ml 1 x 10ml 20X Wash Buffer II 1 x 10ml 1 x 20ml 1 x 10ml 2X Cell Lysis Buffer 1 x 10ml 1 x 16ml 1 x 10ml 8-Well Incubation Tray (with Lid) 1 unit 1 unit 1 unit Biotinylated Antibody Cocktail (C3) 2 vials 4 vials 1 vial Detection Buffer C 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Detection Buffer D 1 x 1.5ml 1 x 2.5ml 1 x 1.5ml Mouse Cytokine Antibody Arrays Membranes (C3) 4 vials 8 x 1l 1 vial -
Research areas
Images
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Multiplex Protein Detection - Mouse Cytokine Antibody Array - Membrane (62 Targets) (ab133995)
Typical results obtained with Abcam Human Cytokine Membrane Antibody Array. These membranes were probed with conditioned media from two different cell lines. Membranes were exposed to film at RT for 1 min.
