Chicken IL-10 ELISA Kit (ab273259)
Key features and details
- Sensitivity: 16 pg/ml
- Range: 14.34 pg/ml - 3500 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Chicken
Overview
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Product name
Chicken IL-10 ELISA Kit
See all IL-10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 10% Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
14.34 pg/ml - 3500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.8 72% - 143% Plasma 86.13 70% - 105% Cell culture media 129.5 115% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Chicken -
Product overview
Chicken IL-10 ELISA Kit (ab273259) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-10 in serum (Chicken IL-10 concentration is low in normal serum/plasma, and may not be detectable in this assay), EDTA plasma (Citrate and heparin have not been validated in this assay yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IL-10 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml Anti-Chicken IL-10 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Chicken IL-10 Antibody 2 vials Chicken IL-10 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all
Images
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Chicken IL-10 ELISA Kit standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
