Cat CRP ELISA kit (ab190520)
Key features and details
- Sensitivity: 0.656 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cat
Overview
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Product name
Cat CRP ELISA kit
See all C Reactive Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum Inter-assay Sample n Mean SD CV% Serum -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.656 ng/ml -
Range
3.125 ng/ml - 200 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 3.125ng/ml - 200ng/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cat -
Product overview
Abcam’s CRP Cat ELISA Kit (ab190520) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring CRP in biological samples of Cats.
In this assay the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of CRP in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Notes
Acute phase proteins are plasma proteins which increase in concentration following infection, inflammation or trauma. C-reactive protein (CRP) is so named because it is able to effect precipitation of somatic C-polysaccharide of Streptococcus pneumonia. CRP is an alpha globulin with a mass of 110,000 to 140,000 daltons, and composed of five identical subunits, which are non-covalently assembled as a cyclic pentamer. It is synthesized in the liver and, in Cats, is normally present as a trace constituent of serum at levels less that 0.3 mG/dL. The levels in serum rise quickly following acute tissue damage and also falls very rapidly once the stimulus is removed. It has been proposed that the function of CRP is to aid in complement activation, influence phagocytic cell function, and augment cell mediated cytotoxicity. Investigations over the past few years have shown that quantification of these in plasma or serum can provide valuable diagnostic information in the detection, prognosis, and monitoring of disease not only in Cats, but in companion animals and farm herds as well.
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Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml CRP Cat Antibody coated microwells 1 x 96 tests CRP Cat Calibrator (lyophilized) 1 vial CRP Cat HRP Conjugate 1 vial Stop Solution 1 x 12ml Chromogen Substrate Solution 1 x 12ml -
Research areas
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Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
Images
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Standard Curve
Representative standard curve using ab190520 (CRP Cat ELISA Kit).
