Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23494-47] to CCR2 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free
    See all CCR2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23494-47] to CCR2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Human PBMC and THP-1 cells. Flow Cyt: Human PBMC and THP-1 cells.

  • General notes

    ab273054 is the carrier-free version of ab254375. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
    Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

    Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
    Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in THP-1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).

  • Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
    Flow Cytometry - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

    Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CCR2 with ab254375 at 1/600 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Cells were stained with rabbit IgG (Left) or ab254375 (Right). Then stained with anti-CD14 conjugated to Alexa Fluor 647.

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
    Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC cells labelling CCR2 with ab254375 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous and cytoplasmic staining in Human PBMC cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254375).

  • Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)
    Anti-CCR2 antibody [EPR23494-47] - BSA and Azide free (ab273054)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.