Carboxylesterase Activity Assay Kit (Fluorometric) (ab273314)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 6.6 µU
Overview
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Product name
Carboxylesterase Activity Assay Kit (Fluorometric)
See all Carboxylesterase 1 kits -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
6.6 µU -
Assay duration
Multiple steps standard assay -
Product overview
Carboxylesterase Activity Assay Kit (Fluorometric) (ab273314) is a simple, rapid, plate-based fluorometric assay for measuring Carboxylesterase activity in biological samples. The kit uses the proprietary substrate for the quantification of Carboxylesterase (CE) activity in samples.
The assay can detect as low as 6.6 µU of Carboxylesterase activity.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests CE Assay Buffer 1 x 13ml CE Positive Control (Lyophilized) 1 vial CE Standard 1 x 50µl CE Substrate 1 x 50µl -
Research areas
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Alternative names
- ACAT
- Acyl coenzyme A:cholesterol acyltransferase
- Brain carboxylesterase hBr1
see all
Images
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Carboxylesterase standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Carboxylesterase Standard Curve (0-500 pmoles/well).
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CE ActivityExample data.
Carboxylesterase activity in human liver microsomes (HLM) (16 mg/ml), and HepG2 lysate (3.21 mg/ml).
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CE activityExample data.
Carboxylesterase activity in HepG2 lysate, human liver microsomes and Positive Control.