Canine C Reactive Protein ELISA Kit (ab157698)
Key features and details
- Sensitivity: 0.6467 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
-
Product name
Canine C Reactive Protein ELISA Kit
See all C Reactive Protein kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
0.6467 ng/ml -
Range
3.125 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Abcam’s C Reactive Protein Dog ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of C Reactive Protein in dog serum and plasma samples.
In this assay the C Reactive Protein present in samples reacts with the anti-C Reactive Protein antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-C Reactive Protein antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound C Reactive Protein. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of C Reactive Protein in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of C Reactive Protein in the test sample. The quantity of C Reactive Protein in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-dog C Reactive Protein antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Dog C Reactive Protein Calibrator (lyophilized) 1 vial Dog C Reactive Protein ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
-
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. -
Tissue specificity
Found in plasma. -
Sequence similarities
Belongs to the pentaxin family.
Contains 1 pentaxin domain. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Pentraxin 1, short
- C reactive protein
- C reactive protein pentraxin related
see all -
Database links
Images
-
Standard CurveRepresentative standard curve using ab157698 C Reactive Protein Dog ELISA Kit