Anti-LC3B antibody (ab51520)
Key features and details
- Rabbit polyclonal to LC3B
- Suitable for: Flow Cyt, IHC-Fr, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LC3B antibody
See all LC3B primary antibodies -
Description
Rabbit polyclonal to LC3B -
Host species
Rabbit -
Specificity
ab51520 is specific for the B isoform of LC3. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-Fr HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human LC3B (N terminal). Synthetic peptide made to the N terminal region of human LC3, isoform B protein.
Database link: Q9GZQ8 -
Positive control
- ICC/IF: HeLa, U373-MGc cells. IHC-P: U87-MG cells. Flow cyt: NTERA-2 cells. IHC-P-Fr: U373-MG cells.
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General notes
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells.
The product formulation changed to include 1% BSA on 17th May 2017. The following lot does not contain BSA and is still in stock GR320876. All future lots will contain 1% BSA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
ab51520 has been shown to work on human malignant brain tumors, breast cancer, leukemia samples, and murine cells. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U87-MG cultured and subcutaneous tumors
1) Control
2) Temozolomide -
Western blot - Anti-LC3B antibody (ab51520)All lanes : Anti-LC3B antibody (ab51520) at 1/3000 dilution
Lane 1 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 0 µM
Lane 2 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 2 µM
Lane 3 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 4 µM
Lane 4 : treated U87-MG (human glioblastoma astrocytoma) lysates with Arsenic Trioxide at 8 µM
Predicted band size: 15 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LC3B with ab51520.An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Flow Cytometry - Anti-LC3B antibody (ab51520)Flow cytometry analysis of NTERA-2 cells with ab51520 at 1/200 using Dylight-488 conjugated goat anti-rabbit IgG secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U373-MG cells;
1) Control
2) C2-ceramide.
The nuclei were stained with DAPI. -
Immunohistochemistry (Frozen sections) - Anti-LC3B antibody (ab51520)Ab51520 at 1/2000 dilution staining treated U373-MG (human glioblastoma) cells
1) Control
2) Temozolomide -
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red) at 0.1 ug/ml. Nuclei were counterstained with DAPI (blue).Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
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Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody (ab51520)Immunocytochemistry/Immunofluorescence analysis of HeLa cells using anti-LC3B antibody(red). Nuclei were counterstained with DAPI (blue).
