Calcium Assay kit (Fluorometric) (ab112115)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell Lysate, Plasma, Serum
- Sensitivity: 0.03 mM
Overview
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Product name
Calcium Assay kit (Fluorometric)
See all Calcium kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate -
Assay type
Quantitative -
Sensitivity
0.03 mM -
Range
0.04 mM - 1.5 mM -
Assay time
0h 30m -
Product overview
Calcium Assay Kit (Fluorometric) ab112115 provides a simple method for detecting calcium in physiological solutions by using a red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em = 540/590 nm.
The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The calcium assay can be completed within 30 minutes.
The calcium assay kit can be used to detect as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).
Calcium assay protocol summary:
- add samples and standards to wells
- add reaction mix
- incubate for 5-30 min whilst measuring the fluorescence intensity with a microplate reader -
Notes
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests 300 mM Calcium Standard 1 x 0.5ml Assay Buffer 1 x 10ml Rhod Red Indicator 2 vials -
Research areas
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Relevance
Calcium is essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% of calcium is found in bones and teeth with the remaining 1% found in the blood and soft tissue. Serum calcium levels are tightly controlled (8.4-11.4 mg/dL) and any variation outside this range can have serious effects. Calcium plays a role in mediating the constriction and relaxation of blood vessels, nerve impulse transmission, muscle contraction, and hormone secretion. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Causes of low calcium levels include chronic kidney failure, vitamin D deficiency, and low blood magnesium levels that can occur in severe alcoholism. -
Alternative names
- Ca++
- Ca2+
Images
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Functional Studies - Calcium Quantification Assay Kit (ab112115)
Ca measured in biologicals showing concentration (mM)
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Functional Studies - Calcium Quantification Assay Kit (ab112115)Standard curve (fluorimetric): mean of duplicates (+/-SD) with background readings subtracted
