Bovine IL-17A ELISA Kit (ab273201)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.05 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Cow
Overview
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Product name
Bovine IL-17A ELISA Kit
See all IL-17A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Quantitative -
Sensitivity
2 pg/ml -
Range
2.05 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 121.1 105% - 136% Plasma 122.5 98% - 149% Cell culture media 130.4 105% - 148% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Cow -
Product overview
Bovine IL-17A ELISA Kit (ab273201) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of bovine IL-17A in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for bovine IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti bovine IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: ELISAmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 250X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Bovine IL-17A coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Bovine IL-17A Antibody 2 vials Bovine IL-17A Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 282863 Cow
- SwissProt: Q687Y7 Cow
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.