Anti-TIPIN antibody - N-terminal (ab236451)
Key features and details
- Rabbit polyclonal to TIPIN - N-terminal
- Suitable for: WB, IP, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TIPIN antibody - N-terminal
See all TIPIN primary antibodies -
Description
Rabbit polyclonal to TIPIN - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human TIPIN (N terminal). ~300 aa.
Database link: Q9BVW5 -
Positive control
- WB: U-2 OS and NIH/3T3 cell lysate. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TIPIN antibody - N-terminal (ab236451)All lanes : Anti-TIPIN antibody - N-terminal (ab236451) at 1/2000 dilution
Lane 1 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with control siRNA cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) treated with TIPIN siRNA cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDaBlocking: 5% TBS-T milk.
Incubated with primary antibody overnight.
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Immunocytochemistry/ Immunofluorescence - Anti-TIPIN antibody - N-terminal (ab236451)2% paraformaldehyde-fixed U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling TIPIN with ab236451 (green) at 1/600 dilution in ICC/IF. Cells were treated with siRNA against TIPIN (right panel) or control (left panel). Counter stained with DAPI (blue). Incubated with primary antibody overnight at +4°C.
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Immunoprecipitation - Anti-TIPIN antibody - N-terminal (ab236451)TIPIN was immunoprecipitated from U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate using 3 µl of ab236451.
Lane 1: U-2 OS whole cell lysate ~400 µg (Input).
Lane 2: Control IgG in U-2 OS whole cell lysate.
Lane 3: ab236451 in U-2 OS whole cell lysate.
For western blotting ab236451 was used at a 1/2000 dilution.
